Plasmid

Part:BBa_J61149:Design

Designed by: John Anderson   Group: Arkin Lab   (2007-09-04)


pBca1101 RIPS reporter plasmid in BBb


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2905
    Illegal PstI site found at 857
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2905
    Illegal PstI site found at 857
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 2905
    Illegal BamHI site found at 1
    Illegal XhoI site found at 851
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2905
    Illegal PstI site found at 857
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2905
    Illegal PstI site found at 857
    Illegal AgeI site found at 561
    Illegal AgeI site found at 673
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1944


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.


Source

pBca9145, synthetic

References